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One unified notebook for your protocols, molecules, inventory, and data.

Protocol execution, chemical inventory, molecular structures, literature citations, and bench DAQ — in one reproducible, audit-ready research environment.

Molecular_&_Biotech_Core/Protocols/PR-2026-ASSAY-04
Encrypted & Cached
STANDARD OPERATING PROCEDUREVersion 3.2·Biochemistry & Analytical Lab

Enzyme Kinetics & Interfacial Impedance Assay

Objective: Quantify substrate turnover kinetics and interfacial double-layer capacitance across functionalized surface substrates.

Phase 1: Reagent Preparation & Inventory DeductionSynced with Lab Inventory
Deducted:Dulbecco's Modified Eagle Medium (DMEM 1X)· 50 mLLot #DM-8891-B
Deducted:Collagenase Type II (0.1% Solution)· 10 mLLot #CL-4012
2Incubation & Cellular Attachment

Transfer seeded scaffolds to 37°C humidified incubator with 5% CO2. Maintain quiescent culture environment.

04:34remaining
Molecular CompoundSMILES: C1=NC(=C2C...
Adenosine Triphosphate (ATP Disodium Salt)
MW: 551.14 g/mol · CAS: 987-65-5
Concentration: 5.0 mM in Krebs-Henseleit Buffer
Reaction Kinetics & ImpedanceMichaelis-Menten / Randles
v = (V_max · [S]) / (K_m + [S])
Substrate velocity modeling & dual-layer electrochemical fit
Target Primer Sequence (Cardiomyocyte Troponin T2)Forward Primer · Tm: 59.4°C
5'-ATGCCTAAGCTGCATGACCGTA-3'22 bp · GC: 50.0%
The complete research environment for modern scientific discoveryTipTap Canvas · LIMS · Citations · Optional DAQ · 21 CFR § 11

Every stage of the scientific lifecycle in one place.

Scientific discoveries happen across wet lab synthesis, molecular assays, mathematical modeling, inventory management, and physical measurements. Inquestra provides native scientific tools for every phase — without stitching together a five-app stack.

Open the workspace

Scientific Protocol Canvas

TipTap block editor with native SMILES structures, nucleotide sequences, KaTeX equations, and live execution timers. Commands: /molecule · /sequence · /math

SOPs & Interactive Step Timers

Reproducible checklists with synchronized incubation, centrifuge, and wash timers. Commands: /protocol · /timer · /checklist

LIMS & Chemical Inventory

Track reagents, antibodies, primers, and cell lines. Inline /reagent deduction automates chain-of-custody at bench execution.

PubMed & Europe PMC Citations

Search peer-reviewed literature in context. Insert verified DOIs and generate formatted bibliographies (Nature, APA, IEEE). Commands: /citation

Audit Trails & Version History

Time-stamped versioning captures procedure edits, reagent calibrations, and author attestation. Standards: 21 CFR § 11 · RFC 3161

Integrated Instrument DAQ (Optional)

Connect biopotentials, load cells, and pressure sensors via W3C Web Serial — no drivers or terminal scripts. Transports: Web Serial · CSV Ingestion

Purpose-built for your laboratory workflow.

Whether you are running PCR gels, synthesizing compounds, testing polymer mechanics, or measuring electrical biopotentials, Inquestra adapts to your domain.

DomainExample workflows & signals
Molecular & Cell BiologyPlasmid cloning, DNA/RNA sequence verification, antibody aliquot management (-80°C), cell culture feeding timers.Primer design · Cell passaging · Western blots
Chemistry & Biochemistry2D chemical structures from SMILES, stoichiometry computation, reagent lot tracking, GHS safety data sheets.SMILES structures · Enzyme kinetics · GHS safety
Biotech & Biopharma R&DBatch records, SOP checklists, real-time deviation logging, 21 CFR Part 11 audit trails with dual-signature sign-offs.SOP management · Deviation logs · 21 CFR § 11
Materials Science & PhysicsTensile test stress-strain curves, load cell deformation recording, continuum mechanics modeling with KaTeX.Stress-strain curves · Load cells · KaTeX modeling
Bioengineering & DevicesBiopotential telemetry (EMG, ECG), hemodynamic waveforms, design history files (DHF) with zero loose CSV files.Web Serial DAQ · Biopotentials · Design history files
Academic Core FacilitiesGraduate student onboarding, shared equipment clearances, institutional research provenance across grants.Equipment tracking · Protocol sharing · Grant archives

Eliminate the five-app shuffle in your lab.

Most research groups waste hours stitching together office document software, unsearchable paper binders, scattered inventory sheets, and separate reference managers. Here is the difference Inquestra makes.

Fragmented Laboratory SoftwareHigh Error Rate
Generic Document Editors (Word, Notion, Google Docs)No native chemistry, molecular structure drawing, or biological sequence support. Equations require clumsy image copy-pasting, and incubation timers exist only on phones or kitchen timers.
Disconnected Spreadsheets for Reagent InventoryChemical lot numbers, antibody aliquots, and buffer volumes tracked in spreadsheets that are never updated at the moment of bench execution, leading to expired stock errors and failed experiments.
Isolated Literature Silos (Zotero, Mendeley, Desktop PDFs)Theoretical rationale and published protocols live in separate citation managers, forcing researchers to manually copy DOIs and lose track of protocol references over time.
Unverified Desktop CSV Files & Terminal ScriptsInstrument data saved to loose desktop folders without cryptographic hashing or audit trails, creating severe reproducibility vulnerabilities during peer review or regulatory audits.
The Inquestra Integrated StandardUnified Research OS
Native Scientific Protocol CanvasSMILES 2D molecular structures, nucleotide color-coded DNA/RNA sequences, KaTeX mathematical equations, and live execution timers integrated directly into notes.
Inline LIMS & Reagent DeductionTyping /reagent automatically deducts volumes from your inventory, checking expiration dates and logging consumption directly in the note.
Direct PubMed & Europe PMC Citation IntegrationSearch peer-reviewed literature in real time. Link scientific rationale, protocol citations, and DOI references directly to experimental steps with automatic bibliography formatting.
Tamper-Evident Record of ExecutionCompleted trials seal protocol text, reagent lots, and raw telemetry into an immutable, 21 CFR Part 11 compliant audit log with non-repudiation.

Regulatory compliance & verifiable integrity.

Scientific trust is earned through verification. Inquestra seals completed trials so protocol procedures, chemical lots, and telemetry samples maintain an unalterable chain of custody.

CRYPTOGRAPHIC TRIAL PROVENANCE
Audit Engine:Deterministic Seal Active

Test Tamper-Evident SHA-256 Binding

Inquestra computes a cryptographic hash over the raw telemetry packet array and the protocol notebook. Modify a single digit to observe how cryptographic verification instantly detects unauthorized post-hoc alterations.

Trial Payload #TR-8841-SCIIndexedDB / Cloud
Recorded Peak Signal Output:1.4820 mV
Reagent Batch Lot:Lot #KR-2026-99
Lead Author Attestation:Instrument Operator [ID: OP-33]
Sealed Digest VerificationPASS: AUTHENTIC
Stored Seal in Immutable Audit Ledger:
8d969eef6ecad3c29a3a629280e686cf0c3f5d5a86aff3ca12020c923adc6c92
Recomputed Checksum of Current Record:
8d969eef6ecad3c29a3a629280e686cf0c3f5d5a86aff3ca12020c923adc6c92

Regulatory Compliance Assurance: Once a trial is locked by the Principal Investigator, all telemetry packets, protocol parameters, and reagent deductions are cryptographically bound. Any institutional auditor or peer-review journal can verify record authenticity with standard SHA-256 tooling.

21 CFR § 11.10(b)

Audit Trails

Computer-generated, time-stamped records independently log the date, time, and operator of all trial additions, calibrations, and seals. Modifications preserve complete differential histories.

21 CFR § 11.50

Electronic Signatures

Manifests display the printed name of the signer, date/time stamp, and the explicit meaning of the signature (Author Execution, Technical Review, or Principal Investigator Sign-Off).

FAIR Data Principles

Local-First & Offline

Full offline continuity inside Faraday cages and basement labs via browser IndexedDB. Export to open formats (CSV, EDF, JSON with cryptographic manifest) with zero platform lock-in.

Optional Hardware Ingestion

When your study needs physical data, connect directly.

If your research involves physical sensors — spectrophotometers, balances, load cells, temperature arrays, or biopotentials — Inquestra can ingest continuous telemetry straight into your notebook note via browser Web Serial. No custom drivers or terminal scripts required.

ESP32-S3 Firmware (C++)

Hardware DAQ

High-speed analog transducer sampling with FreeRTOS ring-buffer queue and USB-CDC telemetry stream.

// Inquestra DAQ Firmware for ESP32-S3 (Arduino Core / ESP-IDF)
// Continuous 1,000 Hz instrument telemetry sampling with hardware timer

#include <Arduino.h>

const int SENSOR_PIN = 1;        // ADC1_CH0 (GPIO 1)
hw_timer_t *timer = NULL;
volatile uint32_t sampleCount = 0;

void IRAM_ATTR onSampleTimer() {
  sampleCount++;
}

void setup() {
  // High-speed native USB CDC at 921,600 baud
  Serial.begin(921600);
  analogReadResolution(12);       // 12-bit ADC (0 - 4095)

  // Configure 1 kHz hardware timer ISR
  timer = timerBegin(1000000);   // 1 MHz base
  timerAttachInterrupt(timer, &onSampleTimer);
  timerAlarm(timer, 1000, true, 0); // 1,000 ticks = 1 ms interval
}

void loop() {
  static uint32_t lastProcessed = 0;
  if (sampleCount != lastProcessed) {
    lastProcessed = sampleCount;
    int raw = analogRead(SENSOR_PIN);
    float voltage = (raw * 3.3f) / 4095.0f; // Scale to volts

    // Emits standard Inquestra CSV frame: [timestamp_ms],[voltage_mv]
    Serial.printf("%lu,%.4f\n", millis(), voltage * 1000.0f);
  }
}
No proprietary libraries or drivers neededStandard ANSI C++ / ES2022 / Python 3
Supported Modalities:Load Cells · Temperature Probes · Biopotentials (EMG/ECG) · Pressure Sensors · Bench Meters
Explore Full Hardware DAQ Specifications

Partner with Inquestra on your bench.

We are onboarding scientific research groups and biotech teams for our pilot program. Work directly with our engineering team to import your existing protocols, calibrate custom instrument profiles, and configure team workspace permissions.

  • — Individual researchers retain full free access to the core notebook, LIMS, and local DAQ tools.
  • — Collaborate directly with the engineers building Inquestra to tailor custom protocol blocks and data export schemas.
  • — Assistance setting up self-hosted institutional PostgreSQL instances, team SSO, and importing existing lab SOPs.

Request Pilot Lab Access & Lab Onboarding

Tell us about your research focus, lab setup, and protocol needs. Inquiries are reviewed within 48 hours.

Free pilot. No credit card. We respond within 48 hours.

Start organizing your research in one unified notebook.

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